Three kinds of bacteria production

Mushrooms such as oyster mushrooms, mushrooms, shiitake mushrooms, and oyster mushrooms are mostly produced and cultivated in summer. Seed production under high temperature conditions tends to result in higher contamination rates and lower yields. In order to ensure the quality of summer and autumn strain production, we must do a good job in the three technologies of sterilization, inoculation, and cultivation.

Sterilization off high temperature in summer, the preparation of raw materials for the medium required fresh, no mildew, use before the sun 1 to 2 days. Stuffed ingredients are sterilized in fashion bags (bottles). When making bagged strains, each production step should be lightly loaded and gently placed. For plastic bags that have been punctured or squeezed, apply plastic sheets or stick them with double bags. The bag mouth can be sealed with a rubber band or tear tape. After bagging, it is sterilized by atmospheric pressure sterilization. The sterilization time is prolonged by 1 to 2 hours depending on the amount of the charged material, and the exhaust gas is slow.

Inoculation vaccination should be carried out in the inoculation box. Vaccination with alcohol lamp flame inoculation, the temperature often reaches 40 ~ 50 °C, very easy to burn or burn bacteria, so the vaccination should be arranged in the morning and evening or during the night when the temperature is low. When inoculated, the volume of each bottle transferred is reduced as compared with conventional production. The inoculum volume should be relatively increased, and the old mycelium in the upper part of the bottle should be removed.

Cultivation of culture-cultivated species requires clean, non-toxic, insect-free, cool, direct sunlight, and relative humidity below 60%. Freshly picked bacteria must wait for the outside of the bag (bottle) and the seal to dry before stacking. It is best to be stacked in "well" shape to facilitate ventilation and facilitate management. In summer, germs grow rapidly, and after 3 days of general inoculation, they should be checked and found to be cleared in time.


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