In nature, there are a large number of microorganisms in air, water, soil, animals and plants, as well as various items. Our preparation of edible fungi strains is to use effective methods to inhibit the growth of other microorganisms and create environmental conditions conducive to the growth of edible fungi. This requires us to disinfect all aspects of the production of edible fungi. Disinfection is divided into three methods: cryogenic disinfection, boiling water disinfection and chemical disinfection.
1. Low temperature disinfection. Low temperature sterilization is to disinfect at a temperature of 60-75 degrees. Low-temperature sterilization can kill the vegetative cells of most microorganisms. For example, the fermentation process of culture materials is not only a low-temperature sterilization process, but also can effectively kill harmful bacteria and pests in raw materials.
2. Boiling water disinfection. Boiling water disinfection is boiling in boiling water for 30-40 minutes to kill the vegetative cells of the microorganisms. To kill spores of bacteria or mold spores, it is necessary to extend the boiling time for more than 2 hours, which is mainly used for the disinfection of metal inoculation equipment, syringes and the like.
3. Chemical disinfection. Chemical disinfection is the use of chemical drugs to kill microorganisms. (1) The disinfectants commonly used for disinfection are: 37%-40% of formaldehyde liquid used for fumigation and air disinfection; 5% of stone carbonic acid solution for spraying ground and wall disinfection; 2%-5 % of bleach solution, 1%-2% of rosul, Bordeaux mixture (1:1 cupric sulfate, lime diluted to 100-200 times the solution) and so on. Note that the operator exhausts formaldehyde as much as possible when entering the mushroom house. (2) Disinfectants used for inoculation room, equipment, mushroom wood surface, etc.: Inoculation, 70%-75% alcohol disinfection of tabletops, equipment, mushroom wood surfaces, inoculation personnel's hands, etc., can not be used anhydrous ethanol and 95% Ethanol. In addition, you can also use 5% of formaldehyde, 0.25% benzalkonium chloride, 0.1% of mercuric chloride solution, 0.1% -0.2% potassium permanganate solution, etc. for disinfection
1. Low temperature disinfection. Low temperature sterilization is to disinfect at a temperature of 60-75 degrees. Low-temperature sterilization can kill the vegetative cells of most microorganisms. For example, the fermentation process of culture materials is not only a low-temperature sterilization process, but also can effectively kill harmful bacteria and pests in raw materials.
2. Boiling water disinfection. Boiling water disinfection is boiling in boiling water for 30-40 minutes to kill the vegetative cells of the microorganisms. To kill spores of bacteria or mold spores, it is necessary to extend the boiling time for more than 2 hours, which is mainly used for the disinfection of metal inoculation equipment, syringes and the like.
3. Chemical disinfection. Chemical disinfection is the use of chemical drugs to kill microorganisms. (1) The disinfectants commonly used for disinfection are: 37%-40% of formaldehyde liquid used for fumigation and air disinfection; 5% of stone carbonic acid solution for spraying ground and wall disinfection; 2%-5 % of bleach solution, 1%-2% of rosul, Bordeaux mixture (1:1 cupric sulfate, lime diluted to 100-200 times the solution) and so on. Note that the operator exhausts formaldehyde as much as possible when entering the mushroom house. (2) Disinfectants used for inoculation room, equipment, mushroom wood surface, etc.: Inoculation, 70%-75% alcohol disinfection of tabletops, equipment, mushroom wood surfaces, inoculation personnel's hands, etc., can not be used anhydrous ethanol and 95% Ethanol. In addition, you can also use 5% of formaldehyde, 0.25% benzalkonium chloride, 0.1% of mercuric chloride solution, 0.1% -0.2% potassium permanganate solution, etc. for disinfection