1. Materials and Methods
1.1 Indigo rice seedlings were planted at the nursery in Yueqing, Zhejiang Province with artificial breeding. In the indoor cement pool, juveniles with an average weight of 460,000 capsules/kg were grown. The average size was 0.221 cm. On July 28, 2002, 2.15kg of seed was transported to the Lubai Center of Jiangsu Marine Fisheries Research Institute, and it was screened and divided into 1 million grains/kg and 400,000 grains/kg.
1.2 Incubation pond A pool of 67m long and 4m wide strips, nurturing water depth 35CM-40EM, sandy bottom; 3 long bars 25m long and 6m wide, cultivating depth 35-40eM muddy bottom .
1.3 Seawater is introduced into the outer sea area and used after secondary precipitation. The change in specific gravity during the test range: 1.011-1.021; water temperature range: 21°C-36.5°C.
1.4 Baits are artificially cultivated Chaetoceros mures, Glycoeyl isobaricum 3011, Heterococcus, Micrococcus sp., D. salina, Pavlova algae and dried yeast.
2. Technical measures and results
2.1 High-density cultivation The cultivation pond A was ploughed into four areas of 50 m2, 50 m2, 50 m2 and 120 m2, and on the 28th of July, the size of 1 million grains/Ks of green barley seedlings was divided into 10000 grains. Density of 6000m2/m2 is broadcasted into two 50m2 areas; 400,000 grain/Kg green barley seedlings are sown at a density of 5000 grains/m2 and 3000 grains/m2 into 50m2 and 120m2 areas, respectively. .
After the green barley seedlings were sowed, they were added to seawater to a depth of 35cm. Drain the water 10cm every day at 8:00 and then add 10cm of artificially cultivated Chaetoceros muelleri or ball algae. An inflated sand head is set for every 4m2 in the pool. During the cultivation period (July 28-September 3), it is filled with air for one hour every morning and noon. During the high temperature period, it is easy to grow furrows, water clouds, etc., and remove them every day.
On September 3, the young plants in each area of ​​A pond were scraped and sieved, and no death was found during the screening process.
2.2 Determination of the growth rate of barley seedlings Since the barley seedlings were placed in pond A on July 28th, the water temperature and seawater specific gravity were measured and recorded regularly every 10 days to measure the average size and weight of the seedlings. On September 3, after the green barley seedlings were scraped from the A pool, they were partially transplanted into the group B ponds that had been previously treated with sediment, and were reared to a density of 2000-2500 seeds/m2.
2.3 Different sediment growth test The experiment was divided into 5 groups with 2 replicates in each group. The test vessel was a plastic round pot with a diameter of 30 cm and a height of 12 cm. The five groups of sediments were: full sand, 1/4 sand + 3/4 mud, 1/2 sand ten 1/2 mud, 3/4 sand ten 1/4 mud, and whole mud. Among them, the sand is a yellow sand with a particle size of 0.5 to 1 mm, and the mud is a mature soil taken from a paddy field. All substrates were soaked in KMnO and placed in a plastic basin after soaking. The thickness of the sediment was 5 cm, and the depth of seawater in the basin was 5 cm. Each of the pots was planted with 30 young seedlings, and their size and weight were measured and recorded.
During the experiment, the temperature range of the water changes from 21°C to 26°C, the specific gravity of seawater is controlled at 1.010, the water in the basin is poured out at 8:00 every day, and then 4cm of precipitated seawater and 1cm of Campylobacter fluosa is added. The trial began on September 29, was cultured for 15 days, and ended on October 15.
2.4 Different bait feed effects The test is divided into 8 groups, each group has 2 repetitions. The test vessel is a round plastic bowl with a diameter of 30cm and a height of 12cm. The test sediments are all argillaceous. After being soaked and disinfected by KMn04, they are put into pots with a thickness of 5cm. The depth of seawater in the basin was 5cm. Each pot was planted with 30 young pods, and their size and weight were measured and recorded.
Among them, the feeds fed by the 7 groups were: Chaetoceros muelleri, Isopia spp. 3011, Heterococcus, Microchicoccus, Salina, Pavlova, and dried yeast. One group was blank and no feed was fed.
During the test period, the water temperature varied from 21°C to 26°C, the specific gravity of seawater was controlled at 1.010, and the water in the basin was poured out every day at 8:00. After adding 3cm deep precipitated seawater, the feed was added separately. The bait feed is based on the principle of exceeding the daily feed intake of the young rice seedlings, that is, there is still algae in the seawater when the water is changed on the second day, and the amount of feed does not become a limiting factor in the test. Among them, the daily dosage of the yeast group was 10x10-6, and the control group only added precipitation seawater daily. The trial began on September 29, was cultured for 15 days, and ended on October 15.
1.1 Indigo rice seedlings were planted at the nursery in Yueqing, Zhejiang Province with artificial breeding. In the indoor cement pool, juveniles with an average weight of 460,000 capsules/kg were grown. The average size was 0.221 cm. On July 28, 2002, 2.15kg of seed was transported to the Lubai Center of Jiangsu Marine Fisheries Research Institute, and it was screened and divided into 1 million grains/kg and 400,000 grains/kg.
1.2 Incubation pond A pool of 67m long and 4m wide strips, nurturing water depth 35CM-40EM, sandy bottom; 3 long bars 25m long and 6m wide, cultivating depth 35-40eM muddy bottom .
1.3 Seawater is introduced into the outer sea area and used after secondary precipitation. The change in specific gravity during the test range: 1.011-1.021; water temperature range: 21°C-36.5°C.
1.4 Baits are artificially cultivated Chaetoceros mures, Glycoeyl isobaricum 3011, Heterococcus, Micrococcus sp., D. salina, Pavlova algae and dried yeast.
2. Technical measures and results
2.1 High-density cultivation The cultivation pond A was ploughed into four areas of 50 m2, 50 m2, 50 m2 and 120 m2, and on the 28th of July, the size of 1 million grains/Ks of green barley seedlings was divided into 10000 grains. Density of 6000m2/m2 is broadcasted into two 50m2 areas; 400,000 grain/Kg green barley seedlings are sown at a density of 5000 grains/m2 and 3000 grains/m2 into 50m2 and 120m2 areas, respectively. .
After the green barley seedlings were sowed, they were added to seawater to a depth of 35cm. Drain the water 10cm every day at 8:00 and then add 10cm of artificially cultivated Chaetoceros muelleri or ball algae. An inflated sand head is set for every 4m2 in the pool. During the cultivation period (July 28-September 3), it is filled with air for one hour every morning and noon. During the high temperature period, it is easy to grow furrows, water clouds, etc., and remove them every day.
On September 3, the young plants in each area of ​​A pond were scraped and sieved, and no death was found during the screening process.
2.2 Determination of the growth rate of barley seedlings Since the barley seedlings were placed in pond A on July 28th, the water temperature and seawater specific gravity were measured and recorded regularly every 10 days to measure the average size and weight of the seedlings. On September 3, after the green barley seedlings were scraped from the A pool, they were partially transplanted into the group B ponds that had been previously treated with sediment, and were reared to a density of 2000-2500 seeds/m2.
2.3 Different sediment growth test The experiment was divided into 5 groups with 2 replicates in each group. The test vessel was a plastic round pot with a diameter of 30 cm and a height of 12 cm. The five groups of sediments were: full sand, 1/4 sand + 3/4 mud, 1/2 sand ten 1/2 mud, 3/4 sand ten 1/4 mud, and whole mud. Among them, the sand is a yellow sand with a particle size of 0.5 to 1 mm, and the mud is a mature soil taken from a paddy field. All substrates were soaked in KMnO and placed in a plastic basin after soaking. The thickness of the sediment was 5 cm, and the depth of seawater in the basin was 5 cm. Each of the pots was planted with 30 young seedlings, and their size and weight were measured and recorded.
During the experiment, the temperature range of the water changes from 21°C to 26°C, the specific gravity of seawater is controlled at 1.010, the water in the basin is poured out at 8:00 every day, and then 4cm of precipitated seawater and 1cm of Campylobacter fluosa is added. The trial began on September 29, was cultured for 15 days, and ended on October 15.
2.4 Different bait feed effects The test is divided into 8 groups, each group has 2 repetitions. The test vessel is a round plastic bowl with a diameter of 30cm and a height of 12cm. The test sediments are all argillaceous. After being soaked and disinfected by KMn04, they are put into pots with a thickness of 5cm. The depth of seawater in the basin was 5cm. Each pot was planted with 30 young pods, and their size and weight were measured and recorded.
Among them, the feeds fed by the 7 groups were: Chaetoceros muelleri, Isopia spp. 3011, Heterococcus, Microchicoccus, Salina, Pavlova, and dried yeast. One group was blank and no feed was fed.
During the test period, the water temperature varied from 21°C to 26°C, the specific gravity of seawater was controlled at 1.010, and the water in the basin was poured out every day at 8:00. After adding 3cm deep precipitated seawater, the feed was added separately. The bait feed is based on the principle of exceeding the daily feed intake of the young rice seedlings, that is, there is still algae in the seawater when the water is changed on the second day, and the amount of feed does not become a limiting factor in the test. Among them, the daily dosage of the yeast group was 10x10-6, and the control group only added precipitation seawater daily. The trial began on September 29, was cultured for 15 days, and ended on October 15.
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