Carbon dioxide incubator operating guide:
First, use 1. Put the supplied pressure reducing valve on the carbon dioxide cylinder, there must be no air leakage at the joint, and the cylinder will not be opened temporarily. Use the hose for the pressure reducing valve output connector (the rubber tube with the outer diameter φ8 and the inner diameter φ4 for the sphygmomanometer) to be connected with the CO2 inlet pipe joint on the back of the CO2 box, and press it with the compression ring to prevent leakage. gas.
2. Wipe the CO2 box working chamber with alcohol, turn on the UV lamp power switch on the back of the box, and disinfect for 1-2 hours.
3. Connect the faucet to the upper left side of the tank with a water pipe. The water jacketed CO2 box operates accordingly!
4. Turn on the power, set the power switch to “1â€, and the green indicator light.
a. The water jacketed CO2 tank must be filled with water first, as follows:
Open the faucet, slowly add water to the water tank, and gradually increase with the water level. When the low water level indicator is off, wait for 3-5 seconds and then stop adding water. At this time, the water level is between low and high water level, and the water level should not be On, the device can be put into operation. When no water is added to above the low water level, the low water level indicator lights up and there is a buzzer sound. If there is too much water in the water, the water will overflow from the overflow of the tank behind the high water level indicator. When the high water level indicator is on, pull out the drain hose on the lower left side and pull out the drain plug to release the water. (Note: Discharge The rubber hose should be straightened about 300mm outward). Until the high water level indicator is off for 2-3 seconds, plug the drain plug again. When the water level alarm is low, water should be added in time, otherwise the temperature controller stops heating.
b. Adjust the temperature setpoint to the desired temperature (eg 37 °C) and the heating indicator (green) should illuminate to indicate that heating is taking place. (Note: Ambient temperature exceeds 25 ° C, air conditioning can be used to reduce the ambient temperature.)
c. Set the CO2 control switch to “0â€. (The solenoid valve is closed, because the solenoid valve will heat up when it is not energized for a long time, which will affect the life of the solenoid valve.)
d. After the temperature reaches the set value. Open the carbon dioxide cylinder. (Before opening, the pressure reducing valve should be loosened as much as possible to prevent the pressure of the pressure reducing valve from being too high, causing the gas hose to burst!) The pressure gauge on the pressure reducing valve indicates the carbon dioxide pressure in the cylinder, slowly Turn the pressure reducing valve knob clockwise. The pressure output of the pressure reducing valve is indicated as 0.05 MPa, and the pointer is in the middle of the scale line. At this time, use the dial to set the carbon dioxide concentration (factory adjusted to 5%). Before turning on the carbon dioxide control switch, turn off the air flow meter and the carbon dioxide flow meter. The carbon dioxide controller displays “1â€, and then there is carbon dioxide entering the inner chamber. As the concentration increases, the CO2 controller displays the CO2 concentration value. When the set value is reached, the device automatically switches to the air and carbon dioxide qi. At this time, the air flow meter float should be adjusted at 760 ml/min (already adjusted at the factory), and the carbon dioxide flow meter float should be indicated at 40 ml/min (factory adjusted); if the float deviates from the above value, Adjust the flowmeter knob to 760 ml/min (air) and 40 ml/min (carbon dioxide) accurately. (After opening and closing the door, there is no flow in the air flow meter in a short time, and the flow rate of the CO2 flow meter is also less than 40ml/min. This is normal)
e. It is generally adjusted to 5%±0.3% carbon dioxide concentration at the factory, which can meet the requirements of users.
f. When the temperature reaches the set value, the fluctuation is within ±0.5 °C, and the carbon dioxide concentration also meets the requirements, the cell culture can be performed. Put in a water tray to make the humidity meet the requirements.
g. Use the machine after the first use of the machine or after long-term use, it should be operated according to the above requirements, and should be checked for contamination inside the box before formal cultivation.
5. Shutdown After the culture is finished, open the culture tray and open the culture dish (remember to see if the distilled water in the water tray needs to be replenished). If the culture is stopped, the CO2 cylinder should be closed first, then the air flow meter and the carbon dioxide flow meter should be turned off, and the CO2 intake switch should be set to the “0†position, and the main power supply should be turned off after the Zui. If it is not used for a long time, the water tray should be taken out and the working chamber should be dried. When the CO2 air inlet switch is turned off, the CO2 box is turned on at 37 °C for 2 hours, the working chamber is dried, and the total power is cut off.
Second, the door temperature adjustment method When the machine is shipped from the factory, the adjustment is based on 37 °C. For example, if the water mist on the glass door in the winter box is large, it is impossible to see the cultured items in the box, and the door temperature knob can be adjusted in the back of the CO2 box. , swivel to the right (ie, in the direction of temperature rise) until you can see the contents of the box; if the actual temperature in the box is higher than the set temperature in summer, turn the adjustment knob to the left (ie, the direction of temperature decrease) ) until the temperature inside the box reaches 37 °C.
Third, the fan adjustment method In use, the size of the air volume will not only affect the uniformity of the temperature inside the box, until it affects the control temperature. If the air volume is too large to affect the cultured item during use, adjust the fan speed knob on the back of the CO2 box until the demand is met.
Cardiovascular:
Relating to the circulatory system, which comprises the heart and blood vessels and carries nutrients and oxygen to the tissues of the body and removes carbon dioxide and other wastes from them. Cardiovascular diseases are conditions that affect the heart and blood vessels and include arteriosclerosis, coronary artery disease, heart valve disease, arrhythmia, heart failure, hypertension, orthostatic hypotension, shock, endocarditis, diseases of the aorta and its branches, disorders of the peripheral vascular system, and congenital heart disease.Related Products:cardiovascular drugs.
Urinary:
For the treatment ofl some diseases of the urinary system.
Different specialists treat urinary system ailments. Nephrologists treat kidney diseases, while urologists treat problems with the urinary tract, including the kidneys, adrenal glands, ureters, bladder and urethra, according to the American Urological Association (AUA). Urologists also treat the male reproductive organs, while gynecologists often treat urinary diseases or disorders in females, including yeast infections. Nephrologists and urologists often work with endocrinologists or oncologists, depending on the disease.
Urinary tract infections (UTIs) occur when bacteria enters the urinary tract and can affect the urethra, bladder or even the kidneys. While UTIs are more common in women, they can occur in men. UTIs are typically treated with antibiotics, according to Dr. Oscar Aguirre, a urogynecologist in Denver.
Incontinence is another common disease of the urinary system. It can come in the form of a pelvic prolapse, which can result in leakage and can be the result of a vaginal
delivery. Then there is the overactive bladder, [which we see a lot and is not related to having children or trauma," Aguirre said. A third condition involves overflow, in which the bladder does not completely empty.
Some common treatments involve medications, physical therapy and pelvic mesh surgery, Aguirre noted. Vaginal laser surgery is also becoming a viable treatment option, he explained. [In another 10 to 15 years, vaginal laser surgery will be another common option for the treatment of urinary conditions."
Interstitial cystitis (IC), also called painful bladder syndrome, is a chronic bladder condition, primarily in women, that causes bladder pressure and pain and, sometimes, pelvic pain to varying degrees, according to the Mayo Clinic. It can cause bladder scarring, and can make the bladder less elastic. While the cause isn`t known, many people with the condition also have a defect in their epithelium, the protective lining of the bladder.
Prostatitis is a swelling of the prostate gland and, therefore, can only occur in men. Often caused by advanced age, symptoms include urinary urgency and frequency, pelvic pain and pain during urination, the Mayo Clinic noted.
Kidney stones are clumps of calcium oxalate that can be found anywhere in the urinary tract. Kidney stones form when chemicals in the urine become concentrated enough to form a solid mass, according to the Cleveland Clinic. They can cause pain in the back and sides, as well as blood in the urine. Many kidney stones can be treated with minimally invasive therapy, such as extracorporeal shock wave lithotripsy, which disintegrates the kidney stones with shock waves.
Kidney failure, also called renal failure and chronic kidney disease, can be a temporary (often acute) condition or can become a chronic condition resulting in the inability of the kidneys to filter waste from the blood. Other conditions, such as diabetes and hypertension, can cause chronic kidney disease, according to the Mayo Clinic. Acute cases may be caused by trauma or other damage, and may improve over time with treatment. However, renal disease may lead to chronic kidney failure, which may require dialysis treatments or even a kidney transplant.
Bladder cancer is diagnosed in about 75,000 Americans each year and is more frequent in men and the elderly according to the American Cancer Society. The symptoms, including back or pelvic pain, difficulty urinating and urgent/and or frequent urination, mimic other diseases or disorders of the urinary system.
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